There was a bug in Pointless for XDS reading due to my
misunderstanding of the STARTING_FRAME value, which is fixed in
version 1.2.13, available from
ftp://ftp.mrc-lmb.cam.ac.uk/pub/pre/pointless-1.2.13.linux (eg)
Otherwise if you send me the file I'll investigate
Phil
On 20 Feb 2008, at
Sabine,
versions of pointless before 1.2.13 have a problem with XDS_ASCII.HKL.
Phil fixed this in 1.2.13, which can be obtained from
ftp://ftp.mrc-lmb.cam.ac.uk/pub/pre/
Why don't you use XDSCONV to go to MTZ format? (and XSCALE for merging)
best,
Kay
Sabine Schneider schrieb:
Hi everyone
Hi everyone,
I have a fine sliced dataset consisting of 720 frames (0.25dg / frame).
I processed them with Xds and used Pointless to convert it to mtz for
putting it in Scala. Spacegroup is P212121 and the resolution ~3.2.
After ~200 frames the Rmerge goes up crazy and after 560 it is OK again
That solved it! I had an older version (1.2.9). Thanks a lot!
Sabine
Phil Evans wrote:
There was a bug in Pointless for XDS reading due to my
misunderstanding of the STARTING_FRAME value, which is fixed in
version 1.2.13, available from
ftp://ftp.mrc-lmb.cam.ac.uk/pub/pre/pointless-1.2.13.l
Hi Juliana,
Since you can purify some protein with IMAC, I suggest that you do a
buffer screening with Thermofluor before running the desalting column
(or gel filtration column) and use the buffer condition(s) that
stabilise your protein for the next purification step and possibly
freezin
Hi All,
I am about to buy a laptop and find that the XP is twice the price of
Vista. Does anyone have positive experience using Vista for
crystallographic packages: CCP4, Coot, O, X-plor? or must I still
avoid it?
Thanks,
Deena
Deena Abells Oren, PhD
Manager, Structural Biology Resource
My Understanding is that XP is twice the price for a reason--Vista has
been considered a major downgrade. It seems that SP1 has not helped.
Of course, there are other options out there besides windows boxes, so
you might also want to investigate those (OS X, Linux). Some of
crystallographer
Or don't buy an OS at all. There are a number of vendors out there that
sell OEM notebooks without OSs. I picked up a Compal IF90 with
WSXGA+ (1680x1050) for a good price.
--paul
Yi Zhou wrote:
I think we are all using Linux, so just choose the cheaper OS (you have
to delete it anyway).
Yi
O
I think we are all using Linux, so just choose the cheaper OS (you have
to delete it anyway).
Yi
On Wed, 2008-02-20 at 15:11 -0500, deena wrote:
> Hi All,
> I am about to buy a laptop and find that the XP is twice the price of
> Vista. Does anyone have positive experience using Vista for
> crysta
Deena,
If you are going to run windows, have to use XP. I tried vista, and the
software wouldn't work.
Scott
On Wed, February 20, 2008 2:44 pm, Paul Paukstelis wrote:
> Or don't buy an OS at all. There are a number of vendors out there that
> sell OEM notebooks without OSs. I picked up a Compal
I have been using CCP4MG, Chimera, O for Windows, Wincoot, and the entire
CCP4 suite (including running Refmac and Phaser through the CCP4i interface)
on my Windows Vista laptop for the past 9-10 months without any issues. It
has worked very well for me, and I have found Vista to be much more stabl
Strange, CCP4 and Coot run just fine on my Vista laptop... Have not tried
all programs though.
Flip
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Scott
Pegan
Sent: Wednesday, February 20, 2008 22:04
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Vist
My attempt was a desktop installation. This was ~1 month ago.
Scott
On Wed, February 20, 2008 3:34 pm, Flip Hoedemaeker wrote:
> Strange, CCP4 and Coot run just fine on my Vista laptop... Have not tried
> all programs though.
>
> Flip
>
> -Original Message-
> From: CCP4 bulletin board [
For those who risk becoming blinded and/or deafened by the vista bells
and whistles:
http://pcworld.co.nz/pcworld/pcw.nsf/feature/1FE803ADF0486D06CC2573990069A75D
"The bottom line: It's Vista's architecture and feature set -- Barth
cited Volume Shadow Copy, Vista's snapshot service, as an ex
Hi all,
sorry I couldn't help it :-)
flames on
http://movies.apple.com/movies/us/apple/getamac/apple-getamac-chooseavista_480x376.mov
flames off
Juergen
James Stroud wrote:
For those who risk becoming blinded and/or deafened by the vista bells
and whistles:
http://pcworld.co.nz/pcworld/p
Hi everyone,
I have been recently relying on XDS quite a bit, but at the same time
worrying about how XDS treats overlaps. We had one dataset that both
HKL2000 and Mosflm would show to have severe overlaps, as expected due
to unit cell parameters and the unfortunate crystal orientation in the
Hear Hear.
OS X wins for me, followed by various flavours of Linux, and then - only
then - a windows box (not running Vista).
--
Dr Antony W Oliver
Cancer Research UK: DNA Repair Enzymes Group
Section of Structural Biology
The Institute of Cancer Research
237 Fulham Road
LONDON SW3 6JB
[EMAIL PR
I am sure Vista is probably just as good (or bad, depending on your
point of view) as XP. Most of this stuff should run just fine (I
understand that Vista is probably loaded with features that will bring
older computer to complete stop, but we are talking about brand new
laptop here). But let
Hi all,
Vista has tons of compatibility issues, especially with opengl. As far as I
know pymol, swisspdbviewer and a number of other opengl based programs do not
work on vista. (Coot has some compatibility problem with Aeroglass, and pymol
might have a stable release for vista soon.) More progr
Dear All,
I have a 3A structure, the quality of the data is not very good. Now I
have refined it to Rfree=40.7 in Refmac.
But it wonnot go further down. Then I used CNS to do annealing, then use
refmac to do rigid body refinement.
The Rfree converged to 0.34, but if I use restrain refinement
Hi Yang Li,
trying alternatives is always good thing to do -:)
If you have latest PHENIX installed, try this:
% phenix.refine model.pdb data.mtz
strategy=rigid_body+individual_sites+individual_adp
simulated_annealing=true optimize_wxc=true optimize_wxu=true
main.number_of_macro_cycles=5
He
Vista or XP, I shamelessly admit that I personally totally fail to
see why - ideology left aside - I would ever buy a *laptop* which is
not a Mac.
Even for the fact that when a Mac is "asleep" you open the cover and
it actually comes up in 1 sec, exactly where it was last evening, its
Postdoctoral positions available
This lab conducts two aspects of research using X-ray crystallography
and other biochemical approaches. One is to understand how a limited
number of transcriptional factors regulate larger amount of genes. Our
model is that two or more transcription factors w
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