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Good day,
we are currently refining a 2.4 protein structure using phenix.refine.
I would like to know how to reduce the adp-variance. We have jumps from 60
to 90 and back between adjacent residues and I do not like it.
I scanned the documentation
Hi Tim
use "wxu_scale" keyword for this. Making it less than the default value
will strengthen the ADP restraints and hence make ADP of adjacent atoms
more similar.
This is mentioned in the Manual although I admit is not too easy to
find. Look for "wxu_scale" in
http://www.phenix-online.or
Hi Tim
Sorry I can't help you with Phenix, I would just like to point out that
from a theoretical standpoint it's very difficult to say a priori what
the value of the ADP variance should be. Since the resolution is 2.4 I
assume you're talking about isotropic ADP's. Essentially all of the
contrib
Dear folks,
While I was running 2fo-fc_map and fo-fc_map in CNS, a error was encountered and aborted the program. In the log file, it said " %XFGRCHK error encountered: Grid in z-direction too coarse (EFT GRID or MAPRes)".
Does anyone know what's going on there and how to deal with it? Thanks.
Best
Hi Jerry,
I believe that if you make your cuvette and sample buffers exactly the same,
you would really cut down on noise, and get an accurate reading. Did you do
that, or is it possible?
Jacob
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Jacob Pearson Keller
Northwestern University
Medical Sci
