Dear Mr. Artem,
I think you can download this style from
http://www.endnote.com/support/enstyledetail.asp?SORT=2&PAGE=1&METH=0&DISC=none&JOUR=structural&BSRT=none&FF1=none&FF2=none&FF3=none&CITE=none&DKEY=714200664522dAA%20%20%20%20%20%20%20%20B
There are other styles for many kinds of journal. I
An NIH-funded postdoctoral position is available immediately for a
highly motivated individual with a strong interest in structure and
function of membrane transport proteins. This collaborative project will
be done in the laboratories of Dr. Jeff Abramson (Department of
Physiology) and Dr.
Hi,
I would like to use the SC program but I m having some problems.
Unfortunately the "examples" webpage is not active anymore so I am
fumbling a bit in the dark.
I can get as far as sc xyzin xxx.pdb
but then it asks for
Selection commands:
and I dont know what to type in.
Can anyone
Hi,
I've tried sending an E-mail to Thomas Schneider as listed on the
HKL2MAP web page in order to get my copy but this guy has not replied in
over one month (holidays?) so I was hoping some of you fellow
crystallographers could provide me with it or point out an alternative
to get it since I
I would like to draw your attention to a protein crystallography position
advertised on the AstraZeneca jobs web pages. This is a permanent position in a
new structure biology team being established at our site in Boston,
Massachussets. The position would suit an experienced, all-round, hands-on
Hi Rosemary,
The SC manual URL http://www.ccp4.ac.uk/dist/html/sc.html does contain
all the information you need, but I agree it takes some deciphering.
The basic parameters are the selections of two 'molecules' whose
interface you want to study. For that purpose, the script or line input
is real
Dear all,
I am trying to crystallize a 7kDa protein with MPD as a precipitant.I got
the needles with precipitation in two days at 30% and in 4-5 days,needles
appear in all the drops(pH 3.8-4.8).The concentration of protein is around
20mg/ml and the volume of mother liquor in 500microlt.What should
One approach is to try modifying your current condition with additives.
We have had some occasional successes with polyol, salt, or solvent
additives modifying crystal morphology from needles to something more
tractable. Hampton research has a good list of 10X additives in their
catalog. In our
Our lab is trying to crystallize a highly soluble (100+ mg/ml) protein
with a molecular weight of 35 kd.
The protein was screened against 1536 conditions at 20 mg/mL. Most drops
were either clear or produced "bubbles" (often oily looking). The few
that had precipitate contained high concentration
Try adjusting the pH close to the pI to reduce solubility.
> From: Yvonne Leduc <[EMAIL PROTECTED]>
> Reply-To: <[EMAIL PROTECTED]>
> Date: Tue, 7 Aug 2007 10:17:18 -0600
> To:
> Subject: [ccp4bb] highly soluble proteins
>
> Our lab is trying to crystallize a highly soluble (100+ mg/ml) protein
Postdoctoral Position in Structural Biology /
BiochemistryWe are looking for a highly motivated Postdoc for
our laboratory at the Institute for Physiological Chemistry at
Ruhr-University Bochum, Germany. Our work involves biochemical and
structural studies on cellular signalling systems with rel
Shivesh,
In addition to the comments that have been made already, I would try seeding
with the needles (or pieces thereof) - macroscopic, or take a couple of needles
and crush them up into really small pieces and use various dilutions of these
very small pieces for seeding. You could do that
Has anyone else noticed that they're only getting some of the postings
on the CCP4 mailing list?
Lately I've been noticing that I often see responses to queries, but not
the query itself. This was further highlighted for me recently when I
never received my own posting back from the mailing l
Yvonne,
Several 'old' proteins have been crystallized from insanely high
concentraitons - concanavalin A for instance can be grown from 100-250
mg/ml solutions by means of 'salting in' using microdialysis. This is of
course highly labor-intensive and also expensive on the protein side.
Look at th
Shivesh,
Having gone down the "needles vs. crystals" route
recently, my two suggestions would be:
1) Lower the protein concentration to 10 mg/ml
2) Slightly lower your precipitant percentage, say to
26%. My situation involved PEG4000, and I noticed
that modifying the protocol to PEG8000 at a sl
First i think that your protein concentration is to low... A common rule is
: protein concentration is good when 50% of your conditions are precipitates
and 50% are clears drops. If most of your drops are clear, i think that you
must increase your protein concentration (a screen from Hampton can he
Dear CCP4,
I have a couple of datasets native and heavy-atom-derivatized. The spacegroup
and cell dimensions of all are the same (or very similar). For some reason
I cannot merge any of the datasets together. If for example I rescale one
native dataset by itself, my Rmerge is about 10%, but
===
Lawrence Berkeley National Laboratory
Software Developer II
===
Software Developer II
Job ID: 20930
Division: Phy
Beam time available @ X6A
http://protein.nsls.bnl.gov
The X6A NIGMS beam line at the NSLS allows for fast access to beam time at any
time of the year.
Check availability and schedule your own time directly on the web, as easy as
1, 2, 3, ...
Next available time August 11th.
For comments or f
Reductive methylation of amino groups (lysines and the N-terminus) is
a fairly routine chemical modification that should drop the solubility
of your protein. An easy-to-use protocol can be found in Walter et al
(2007) "Lysine Methylation as a Routine Rescue Strategy for Protein
Crystallization."
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