Dear CCP4 Readers,
Quite some time ago, before the CCP4BB changed its server, I
posted the following inquiry concerning high B-factors in a protein-DNA
complex from a twinned crystal in which most of the DNA was not visible.
Dear CCP4BB:
We have a protein-DNA complex from a twin
Dear All,
I can't seem to read my mtz files with 'PHASER for MR' in version
ccp4i 6.0.2 running on either a linux or irix system. I can read the mtz
files with virtually every other CCP4 subroutine. Any ideas/suggestions?
The error I get in the log file is "
*
Detwinning data and refining against detwinned data is not the best
thing to do, especially when you realise that detwinning data with
twin fraction larger than 45% is done using model information in CNS:
the detwinned data you get out is not experimental anymore.
Subsequently, you cannot trust yo
Hi,
this looks very familiar to me. I am pretty certain you have phenix
installed, too, and ccp4i does use the phenix version of phaser, which shows
this behaviour.
You can test this by typing 'which phaser' from the command line.
You should make sure that $CBIN comes before the phenix path, e.g
Wouldn't it be reasonable to use the sigma one calculates from the
sigmaA? That sigma would reflect the uncertainty in the calculated
structure factor amplitude due to the uncertainty in the parameters
in your model. Of course, one then realizes that you should down
weight you structure facto
