[ccp4bb] ICCBM12 conference , Cancun Mexico

Dear collegues, I would like to bring to your attention *ICCBM12*, the 12th International Conference on the Crystallization of Biological Macromolecules, that will take place in Cancún, Mexico (May 6-9, 2008). The conference will be preceded by two day, hands on, crystallization workshop. P

Re: [ccp4bb] AW: [ccp4bb] removal of sulfate ion from the active site

Exactly, solving the equation is necessary to estimate the required concentration Set up the eqn., use large volume (1mL) for soaking solution and small volume (1microL) for the crystal: therefore C(free) ~ C(total), simplify, solve for bound ligand in crystal. Thats all. And I can tell you: st

[ccp4bb] Stereo glass with LCD monitor...

Dear Members, We recently purchased a DELL graphics workstation with LCD monitor (17"). Till now I had assumption that stereo glass will not work on LCD monitors and it needs CRT monitors. But a company with in the following link https://edimensional.com/product_info.php?cPath=21&products_id=28&

[ccp4bb] Bernal Symposium. Dublin, Ireland. 3 -4 September 2007

Bernal Symposium. Structural Biology Dublin, Ireland 3 - 4 September 2007 A symposium to honour the many contributions of J. D. Bernal (1901-1971) to structural biology will be held in Dublin, Ireland, 3rd and 4th September, 2007. Among the participants are individuals who knew or

[ccp4bb] Post-doctoral position

I currently have an open post-doctoral position for a project to crystallize parasite proteins as part of a drug discovery effort. For anyone interested in applying please email a copy of your cv and three letters of reference to the address below. --- Me

[ccp4bb] crystal shipping at room temperature

Dear all, Sorry for the non CCP4 questions. We would like to ship some virus crystals to a synchrotron at room temperature (for room temperature diffraction). I am wondering if anybody has ever had any good experience for this kind of shipping. Especially, it would be great if anybody has any g

Re: [ccp4bb] crystal shipping at room temperature

Hi Junhua: These are ccp4 bb questions, so don't apologize (or we all will start having to do so). All of my room-temperature shipping experiences are pre 9-11-2001, and involve carrying stuff with me on airplanes, which is now an impossibility (unless you possess a Saudi diplomatic pouch, but in

Re: [ccp4bb] crystal shipping at room temperature

Hi Junhua & Bill, I wonder about growing crystals in some kind of gel. Long back, i received the crystals in gel, and they did diffract. Is it still in practice ? regards Manish William Scott <[EMAIL PROTECTED]> wrote: Hi Junhua: These are ccp4 bb questions, so don't apologize (or we all wi

[ccp4bb] How to determine ligand binding from diffraction pattern?

Dear all, Is there a simple way to determine whether ligand is bound or not by comparing the diffraction patterns between ligand-free (structure known) and ligand-soaked protein crystals? I would like to solve the ligand bound protein structure, but before I do so, I have to find out if the lig

[ccp4bb] A babyish question on coot

Hi, I use coot almost around the clock. One thing troubles me is that: when clicking on 'real space refine zone', coot seems only care about the 'real space'(Electron density), sometimes it will bring the model to the density no matter whether the density was already claimed by other model at

Re: [ccp4bb] A babyish question on coot

Try alternate rotamers first, and then choose the closest to the 'right' electron density, before you do 'real space refine' Regards, Josiah. Hi, I use coot almost around the clock. One thing troubles me is that: when clicking on 'real space refine zone', coot seems only care about the 'real s

Re: [ccp4bb] A babyish question on coot

Optimize zone is also a good choice. Just drag it to the right conformation. Hold Ctrl to drag only one atom. If you include a region for real space refinement, there will be no clashes between amino acids in the region. Nian On 5/28/07, Yanming Zhang <[EMAIL PROTECTED]> wrote: Hi, I use coot

Re: [ccp4bb] crystal shipping at room temperature

Hi Junhua, you should contact TSA in Houston, speak to them prior to your flight arrange a contact person who will be there when you are traveling. Send them a Fax explaining what and why you are carrying your crystals with you and how e.g. small cooler with pads etc. I carried some RT 96 well

Re: [ccp4bb] crystal shipping at room temperature

I know this is not what you ask for, but I have had good experience with mounting in capillaries (borosilicate, a bit stronger than quartz, but quartz should work also if you are very careful), leaving the crystals in the mother liquid, to fly them over by FEDex from USA to Europe. Leave the cap