Re: [ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

we were also once able to change 1.7 M Amm sulphate to about equal ionic strength of citrate, in a few concentration steps of mixtures (for other reasons, it was supposedly an inhibitor..).you probably can switch to some friendlier salt... plus you could combine that with gentle crosslinking throug

[ccp4bb] Rfree continues to decrease while Rcrys is more or less static

Hi all i m trying to solve a structure at 2.2A using MIR. i obtained phases using solve. density modification n model building was carried out using phenix. i m solving structure in p212121 space group, with 4 fold NCS. i got almost 80% model built using phenix, rest of the model was built using