Dear CCP4bbers,
We are pleased to announce the (slightly delayed) third anniversary
release of coot:
source: http://www.ysbl.york.ac.uk/~emsley/software/coot-0.3.tar.gz
binaries: http://www.ysbl.york.ac.uk/~emsley/software/binaries/stable/
Happy Easter.
Paul.
Release 0.3 "Basil Grande"
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Hi all,
I keep my protein at 1 M NaCl, at some stage of purification, for about 30
minutes. Protein does not
precipitate out at that stage and susequently when the salt concentration is
reduced. I would like to know
if such a high salt concentration can perturb ( irreversibly) the structure or
You have to judge using some sort of a biochemical or biophysical method.
Yes, protein can be functionally/structurally impaired and yet still soluble
- however there is very little reason to suspect this due to salt exposure -
unless you have hard evidence.
I have worked with several proteins tha
