Re: [ccp4bb] very high concentration of protein

2009-07-02 Thread Filip Van Petegem
Dear Peter, it's a common phenomenon to create protein concentration gradients inside a protein concentrator. Simply take it all out of the concentrator, put it in a separate tube, and mix thoroughly/vortex. The 'slime' may very well redissolve and you'll a homogeneous distribution. What you des

Re: [ccp4bb] very high concentration of protein

2009-07-02 Thread Artem Evdokimov
Peter, If I understand what you are saying - then it is very likely that your protein forms aggregates. Whether this happens on concentration or not is unknown because concentration may simply bring the pre-existing aggregates to the membrane. You can try to make concentration process 'easy