Dear Peter,
it's a common phenomenon to create protein concentration gradients inside a
protein concentrator. Simply take it all out of the concentrator, put it in
a separate tube, and mix thoroughly/vortex. The 'slime' may very well
redissolve and you'll a homogeneous distribution. What you des
Peter,
If I understand what you are saying - then it is very likely that your
protein forms aggregates. Whether this happens on concentration or not is
unknown because concentration may simply bring the pre-existing aggregates
to the membrane.
You can try to make concentration process 'easy