I'll also recommend Buccaneer. You might try using a combination of
PARROT for density modification and NCS averaging followed by
autobuilding with BUCCANEER using initial phases from your MR solution.
You only have two copies of the protein in the ASU, so you only get a
modest boost in electro
Hi Vikram,
Try Buccaneer, it works much better at such resolution than Arp/Warp.
Launch it several times with different parameters (e.g. use original
structure as seed/initial/nothing), then open the results together and
merge the pieces that look best and have lowest b-factors. Use Buster
fo
Well - it sounds hopeful but still challenging..
First suggestion - are you SURE the SG is C2221 and not C222?
If your two molecules are related by a NC translation of x_tran, y_tran,
1/2 then you will get the apparent absences along the 0 0 l axis which
suggest SG C 2221 but the true SG could be
Hi Vikram,
lots of questions for you:
- what do the images look like? Were there “contaminating” lattices? Did the
spot shape look ok? Do they get worse later in the data collection, i.e. do you
have radiation damage? Is the image quality regular or worse at certain angles?
- how good is the dat
Hi everyone,
We are trying to solve a protein structure of 2.6 A. We have processed it
with HKL2000. We have even tried processing with mosflm and xia2. It is in
C2221 space group (checked by pointless) and data is not twinned. It has
31% identical with a search model and has 57% sequence coverage
