Hi Raya,
My reply comes late but maybe it is still relevant to you. I have a few
suggestions for molecular replacement in phaser:
* Input both protein models in your search (define both known structures
as ensembles and search for both)
* Give higher values (let's say 1.0, 1.2, 1.4) for the "rms
Hi,
- I think someone already pointed out that you should try P6522.
- check that it isn't really a twinned P61 or P65 with 2 per asymmetric unit
- buy DNA with BrdU and some more with IdU, and/or grow your protein
in SeMet - at your resolution, the more "real" phase info the better!
Pho
Raja Dey wrote:
Hello,
I am trying to solve a multi-protein DNA complex structure from a 3.6
A native data set. The target structure is a dimer (95 aa in each monomer) in
complex with DNA( 15 base pairs) plus a second protein of 131 aa. The data has
been scaled to P6(1)22 sp. gr. and
