Hi, Tassos,
It appears I am no better a biophysicist than you are on this one (I can
see my Scattering instructor from grad school disapproving of me right
now, he reads this bb). I cannot connect how the laser-induced dipole
and concentration-dependence of refractive index of your sample woul
ct: Re: [ccp4bb] off topic - static laser light scattering
Dear Tassos,
Your assumptions are right, if (1) your dn/dc is accurate, or (2) your
machine is calibrated. We recently measured a protein of a similar size to
yours, and when a 700 Da ligand was added to the buffer, the measured
p
Dear Tassos,
Your assumptions are right, if (1) your dn/dc is accurate, or (2) your
machine is calibrated. We recently measured a protein of a similar size
to yours, and when a 700 Da ligand was added to the buffer, the measured
protein mass was increased accordingly. So MALS can be pretty acc
Tassos,
The most obvious answer (and possibly incorrect) is that DNA itself has a
different dn/dc value and when you say that you have a DNA binding protein,
chances are that some or all of it may be bound to DNA, which would change the
nature of the beast (and the MW). Perhaps you can delibe
