Plus check the insoluble part or the whole cell.
Date: Sat, 5 May 2012 16:30:06 -0400
From: jubo...@jhsph.edu
Subject: Re: [ccp4bb] no expression
To: CCP4BB@JISCMAIL.AC.UK
Some very stupid questions from my side:- it is an expression vector and you
are in frame with your gene of interest
Some very stupid questions from my side:
- it is an expression vector and you are in frame with your gene of interest ?
- you are using E. coli strains suitable for expression ?
- have you tried adding glucose to the media to avoid leaky expression in case
your protein is toxic to E. coli ?
- the
