Chris
Isopropanol is said a very good precipitant but it's unpopular because it's
so difficult to harvest crystals
However there's a very convenient way to grow and harvest crystals in
isopropanol using Vapor Batch plates.
Basically you set up microbatch-under-oil drops containing all the
ingred
In the instance where we grew crystals is isopropanol, we found they also
grew in PEG6000. The best crystals grew in isopropanol, so we just
harvested the crystals into a similar PEG6000 solution. I would imagine
that you can get the crystals to grow in something beside isopropanol
especially now
Nat,
A few years ago I had K channel crystals that formed under similar
conditions. I found that using MPD as a cryo-protectant worked best. As
for the evaporation issue, I had a little extra time as I was performing the
mounting in a cold room.
Scott
Pegan S, Arrabit C, Zhou W, Kwiatkowski W,
Yes, oil is great, but you have to be careful to choose an oil in which
the alcohol is not soluble, or the oil will suck it out of your drop,
(just like air). This is particularly annoying with detergents, which
are almost all soluble in oil. I've always thought that maybe some
synthetic moto
I have wondered if placing a layer of oil over the drop would help
solve the problem of the crystals moving around. Haven't tried it,
but don't people harvest from a microbatch tray by dragging the loop
and crystal through oil?
Nat
On Fri, Apr 9, 2010 at 11:21 AM, James Holton wrote:
> Yes, iso
Yes, isopropanol is a cryoprotectant, and a relatively good one. So are
the other alcohols. It was even popular in the "olden days" when we
would typically set up drops that were 5-10 microliters in volume
(each!). These take a while (minutes) to evaporate, giving you enough
working time to
Working with isopropanol at room temperature and in a well ventialted
environment is a nightmare. Everything moves around wildly until all the
isopropanol has evaporated. Simply leaving the plate in the cold room for a
couple of hours or overnight, and then doing all the mounting in the cold
room a
Dear Chris,
I recall from many years ago that we had a screening hit in high
isopropanol, which started boiling violently when we opened the well. We
"solved" the problem by using another precipitant but I would also be
interested in tricks how to handle high isopropanol.
Best regards,
Herman
