A really simple trick is to dilute your drops.
This has worked to suppress excessive nucleation for us many
times, and nearly always increases crystal size. For example,
instead of setting drops with 1 uL of protein and 1 uL of well
solution, try setting the drop
Dear Eswar,
You could try to slow down crystallization somehow. There are several
ways to accomplish this:
1) increase drop size
2) add small amounts of glycerol (1-5% v/v)
3) cover the reservoir solution with paraffin or silicon oil or a
mixture of both in order to reduce the vapor-diffusion rate
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Dear Eswar,
you can not only alter the protein concentration but also the
precipitant concentration. If your protein really is soluble before you
set up the crystallisation trials, you are bound to find a condition
without crystals by lowering the pre
