Phoebe,
We were able to purify the estrogen receptor DNA binding domain with a
6his-tag. After cutting off the tag and re-applying to the Ni-NTA matrix,
the protein did not stick to the beads. We had some low resolution crystals
that contained a second protein and DNA, which suggests to me that th
Phoebe,
It just so happens that we've been working on a C4 Zn-finger (or something
very much like a Zn-finger) very recently without knowing that we had one
(it's in the PheRS from S. haemolyticus, link to paper below) and we've
purified the protein w/o any issues on an IMAC column (His-SELECT). W
