The main issue is that carboxyls seem to be invisible and Coot tries to fit
them as though the map had them there
Well, if the atoms are part of the model, then Coot will try to fit them to
the "data." I routinely chop off GLU and ASP side-chain when modelling into
cryo-EM maps (that's what th
On 01/02/2017 17:11, Trevor Sewell wrote:
I have a 3.4 A (enzyme – protein only) map that I have fitted manually using
Coot and
automatically with Rapper. It all looks very nice – I can fit all but 13 of the
330
residues. I have the following questions:
Is there a way of having Coot know tha
Dear Trevor,
I do not know if you are aware of it but there is a different version of refmac
which is just made for Cryo-EM refinements.
(http://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/software.html)
In there, Refmac reports an average FSC value which is way more useful than the
R-facto
