I would recommend any one of these things:
1. Increase the concentration of the chemicals present, i.e. add more
iso-propanol OR add more PEG, but use a low molecular weight, like PEG400.
Either one or both. You will need 5% glycerol to get a good (ice-free)
cryo-condition or 10% PEG400 or 10
Dear Yang Li,
Cryoprotection of crystals is not an exact predictive science - you have
to try a number of things, and in the worst case scenario, none of the
stuff you try may work out.
Assuming that your crystals are OK to start with (capillary mounted room
temp. pattern would confirm that), you
