Hi Firdous,,
As you mentioned they are many things to change and try.
before changing vector it is more efficient in my opinion to change
expression conditions (temperature, amount of IPTG, length of culture),
culture medium (regular ones against M9 or auto induction media) and
expression strains
Hey Firdous
Yes, too much information can be confusing. I have expressed and purified
over dozen of different proteins. I usually found in literature the
protocol for the expression and purification of another homologous protein.
That is the best way to start in my opinion. Some folks may want to
