Hi Ed,
A bit late on the subject
I have collected atomic resolution data (around 0.97A) on both bovine
and porcine phospholipase A2 crystals which at
the time of data collection were between 10-16 years old.
Crystallization setup was liquid-liquid diffusion in glass capillaries.
Diffe
I have to second that.
I recently had crystals that will only grow after seeding and will live
for exactly five days. On the sixth day, the same drop will have tracks
of dissolved crystals left in every drop: they almost look like tire
tracks. The crystals frozen on the fifth day diffract to 2.
For a counterexample, from Iwata's group,
Horsefield et al. Succinate: quinone oxidoreductase Acta. Cryst.(2003). D59,
600-602:
"It proved critical to freeze the crystals within 72 h of crystallization set-up. Crystals that were frozen after this time limit
showed no diffraction. This alteratio
FWIW, many of the crystals used in "classical" neutron diffraction
experiments were pretty elderly samples by the time data collection was
initiated, partly to allow large crystals to grow ever larger, partly
because of the mandatory deuterium exchange process and partly because the
experiments las
As written in
Jovine, L., Djordjevic, S. & Rhodes, D. (2000). “The crystal
structure of yeast phenylalanine tRNA at 2.0 A resolution: cleavage by
Mg(2+) in 15-year old crystals” J Mol Biol 301, 401-414.
"Furthermore, the possibility of a Mg2+-catalysed cleavage of the
phosphodiester bond
I had a structure that was done with crystals that were about a year
old. Initial crystals appeared in a less then a day and diffracted
very poorly. In trying to make room for more trays I reexamined the
old trays before throwing them out and low and behold nice well
diffracting crystals.
Here's another very similar case:
http://www.ncbi.nlm.nih.gov/pubmed/12270703
On Thu, Feb 5, 2009 at 11:48 AM, William G. Scott <
wgsc...@chemistry.ucsc.edu> wrote:
> Some things improve with age. Here is one of my favorite stories:
>
>
>
> http://tinyurl.com/oldtrna
>
>
> The crystal structure o
Some things improve with age. Here is one of my favorite stories:
http://tinyurl.com/oldtrna
The crystal structure of yeast phenylalanine tRNA at 2.0 Å resolution:
cleavage by Mg2+ in 15-year old crystals
Luca Jovine, Snezana Djordjevica and Daniela Rhodes
We have re-determined the crys
