Hi Andrea,
If you haven't already done so it might be worth trying a room temperature
mount (capillary or in-situ) to get a feeling for how well the crystals
diffract to start with.
Dave
David Hargreaves
Associate Principal Scientist
Dear Andrea,
as others have suggested there are many ways to dehydrate crystals
by increasing the concentration of salt/precipitant or adding
glycerol/EG/PEG 200-400 to the solutions surrounding your crystals. I
have always found controlled dehydration using a specific device to be
much
Dear Andrea
check out:
Post-crystallization treatments for improving diffraction quality of protein
crystals.
Heras B, Martin JL.
Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1173-80.
All the best
Savvas
On 26 Aug 2011, at 22:53, Andrea L Edwards wrote:
> Hi all,
>
> What are the mos
Andrea L Edwards wrote:
Hi all,
What are the most successful methods you know of for dehydrating a crystal
prior to freezing it? I am trying to push the resolution of my crystals.
Thanks,
Andrea
First of all, be aware that not all crystals are improved by
dehydration. Some need to be drier,
