(A tad delayed)
Actually, typically we set up crystal trials for both cleaved and
uncleaved protein right away at the same time (if there's enough protein
and the cleavage works).
Cheers
phx
On 07/01/2011 15:10, Bosch, Juergen wrote:
I vaguely remember a paper by Aled Edwards, where they did
The RCSB PDB site (www.pdb.org) provides as sequence motif search as an
advanced search option. For example to search for a N-terminal (His)6 tag, use
the following regular expression:
^HH
or HH to find any sequence with a (His)6 pattern.
Click on this link to retrieve all seq
I vaguely remember a paper by Aled Edwards, where they did the statistics for
their targets at SGC. As far as I recall they engineer with cleavable tag
purify and cyrstallize it first with tag and only if they don't get crystals or
a structure they cleave off the tag and rerun the screens. I thi
