Dear Meg,
Please note that there are many ways to do HIC. Different resins result in
different degrees of separation and it's unfortunately impossible to predict
what would happen without trying it out. Which is why various companies sell
HIC 'trial kits' - a few little columns packed with media s
Hi Meg,
ethanol is often used in a HIC elution buffer as it is good at
breaking hydrophobic/hydrophoic interactions. (Detergents or
isopropanol can be used to do the same thing). But, as with all
things, tolerance to ethanol depends upon your protein.
At pH 5, I would guess that Citrate or Acetat
