Re: [ccp4bb] How to generate a complete viirus PDB

2013-04-25 Thread Cathy Lawson
Dear Hong, As Eugene mentioned, you should be able to build the full virus assembly using PISA. Another option is to use the multiscale model menu of UCSF Chimera (default setting is "Biological Assembly") to generate the complete virus assembly from PDB BIOMT records. If this is a new struct

Re: [ccp4bb] How to generate a complete viirus PDB

2013-04-25 Thread eugene . krissinel
Would PISA not do this? -- Eugene On 25 Apr 2013, at 09:20, abhimanyu singh wrote: Hi Hong, In your PDB file if the BIOMT transformation matrix (under remark 350) is provided, you can use this script to generate the biological assembly : http://watcut.uwaterloo.ca/cgi-bin/makemultimer/ Greeti

Re: [ccp4bb] How to generate a complete viirus PDB

2013-04-25 Thread Bradley Kearney
ology Jack Johnson Laboratory From: sujata halder [mailto:halder.suj...@gmail.com] Sent: Thursday, April 25, 2013 12:32 AM To: Bradley Kearney Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] How to generate a complete viirus PDB Hi, In continuation to the previous suggestion about usin

Re: [ccp4bb] How to generate a complete viirus PDB

2013-04-25 Thread abhimanyu singh
Hi Hong, In your PDB file if the BIOMT transformation matrix (under remark 350) is provided, you can use this script to generate the biological assembly : http://watcut.uwaterloo.ca/cgi-bin/makemultimer/ Greetings, Abhi On Thu, Apr 25, 2013 at 9:31 AM, sujata halder wrote: > Hi, > > In continu

Re: [ccp4bb] How to generate a complete viirus PDB

2013-04-25 Thread sujata halder
Hi, In continuation to the previous suggestion about using the 'Oligomer Generator' in viperdb. To use viperdb, your pdb needs to be in the viper format. So download any pdb (example this one : http://viperdb.scripps.edu/cgi-bin/viper_coord.cgi?VDB=1z14) and then superpose your monomer onto this r

Re: [ccp4bb] How to generate a complete viirus PDB

2013-04-24 Thread Bradley Kearney
Hi Hong, I would recommend that you check out the services at the VIPERdb website http://viperdb.scripps.edu. It looks like the oligomer tool (http://viperdb.scripps.edu/oligomer_multi.php) might be useful for what you want to do. They have many of the virus structures from the PDB already proc

Re: [ccp4bb] How to generate a complete viirus PDB

2013-04-24 Thread Matthew Bowler
Hi Hong, you can usually download the 'biological assembly' from the PDBe, be warned that for viruses this is rather large, cheers, Matt. On 25/04/2013 08:19, Guan Hong Hsiang wrote: Dear all: I have one subunit PDB of one virus with a NCS record. Which program should I use to gener