Re: [ccp4bb] Helium-Temp Cryo-Cooling

2017-04-04 Thread Jan Kern
Hi Jakob, There are several other examples where the effect of temperature on radiation damage for metallo proteins was demonstrated, eg: 10.1074/jbc.M112.438796 10.1073/pnas.0505207102 10.1074/jbc.M509724200 The tricky part with liq He cryo streams is to get a good geometry that provides stable

Re: [ccp4bb] Helium-Temp Cryo-Cooling

2017-04-04 Thread Colin Nave
Jacob Good question. One case I know is for a metalloprotein where the photoreduction of the metal centre is decreased significantly at 40K compared with 110K. Acta Cryst. (2007). D63, 951-960 http://scripts.iucr.o