Dear Atul,
Whilst finding Se-Met conditions you could always just phase with mercury if
you are lucky enough to have an accessible free Cys residue or try NaI or I3C
soaks. Always worth trying if you have readily reproducible crystals…
Best of luck
Marc
From: atul kumar mailto:atulsingh21...
Don't forget to do seeding with the native crystals: it's often more about
nucleation than growth. Phx
Sent from tiny silly touch screen
- Reply message -
From: "Frederic VELLIEUX"
Date: Mon, Jun 13, 2011 07:37
Subject: [ccp4bb] Fwd: Regarding Sel-Met containing proteing crystallisatio
日期﹕ 2011/6/13 (一) 2:37:10 PM
主題： Re: [ccp4bb] Fwd: Regarding Sel-Met containing proteing crystallisation
I've had exactly the same with an enzyme from Trypanosoma brucei, native enzyme
gives ca. 2 A data, a search around the crystallization conditions for the
Se-Met
enzyme returns absol
I've had exactly the same with an enzyme from Trypanosoma brucei, native enzyme
gives ca. 2 A data, a search around the crystallization conditions for the
Se-Met
enzyme returns absolutely nothing. What I should do is to search for new
crystallisation conditions (which I won't do: trypanosome me
