Using an envelope or a mask to start phasing even with high NCS is still worse
than using a low res model from cryoEM that is characterized by an (almost)
proper density distribution.
One has to measure the intensities of the very low res reflections (0 0 1 and
the likes) precisely.
The presen
Dear David,
I would remind that the "molecular-replacement positionning" of molecular
envelopes and some methodological features (in comparison with a conventional
MR) of this have been discussed in :
Urzhumtsev & Podjarny (1995). "On the Solution of the Molecular Replacement
Problem at Very
Hi Francis,
As you may have guessed - my question is stems from my recent
acquisition of some native data for a protein that I have lots of SAXS
data for.
I suspect that more conventional MR is unlikely to work in this case
as I only have a half-decent phasing model for ~30% of the protein (2
cop
Point 3:
Use the saxs model for preliminary phasing of your HA derivative
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD
Hi David -
I think you have four hurdles to overcome:
1. How good is a SAXS envelope
2. How will you place it in the right place
3. How will you extend from ~15-20 A to around 4 A
4. How will you extend from 4 A to beyond
Steps 2 and 4 might be the easiest - albeit far from trivial.
Point 1 can
