Hi all,
Just to mention a side-comment relevent to Prasenjit's question:
Considering the prevalence of synchrotron-radiation-damaged disulfides, well
documented in the
literature, it seems that all disulfides in proteins whose data were collected
at synchrotron
sources should be modeled with tw
It seems odd from energetic considerations that there would be a
free-form amino acid when it "has a choice" to be involved in
energetically favorable interaction. It sounds doubly odd in the case of
a cystein involved in a disulfide bond.
Could it be that you are observing the effects of radiation
The SSBOND record does not allow the specification of
an "alternate location indicator". The PDB practice is to
list the SSBOND record if any confirmation is in an SS-bond.
I think that refmac has problems with this since it will
try to apply the SSBOND patch to both confirmations. The
workar
