Dear Niu,
It is very unlikely that MBP will be disordered. We use that protein a
standard for SAXS calibration and gel filtration it is extremely well
behaved.
There is an excellent review about MBP enhanced crystallization (by Moon et
al) and you can do a quick survey of the PDB to find the corn
Dear Randy,
Over the last ~6 years, we have tried to use the MBP tag to enhance
crystallization on >10 proteins. Only one that couldn't crystallize by
itself in the end crystallized with the fusion tag. However,
unfortunately, the target protein (~10 kDa) turned out to be in the big
cavity of the
Dear Niu,
When it works, crystallising a fusion protein can be great, with the big
advantage that placing a model for the (known) carrier protein gives free phase
information. Certainly there are examples of this working, but in the early
days of this (20 years or so ago?), I remember hearing
Hi Niu,
Several things come to mind. First, it may not be trivial when the first
component to be placed is ~20kDa and the second component (SU) is ~43kDa.
The signal after placing the first component may be weak. Also, if the
model for the smaller SU has low sequence identity with the target and y
