Hampton makes a "Detergent Screen" that works the same way as the Additive
Screen.
http://hamptonresearch.com/product_detail.aspx?cid=1&sid=39&pid=31
It has 96 unique detergent reagents for crystal screening.
It is a bit expensive, but if you don't want to purchase the whole kit you
could ju
Rashmi,
Yes, you should use the needle for seeding.
The first thing to do is to seed your control drop to make sure that the
needles
are not bicine, as this buffer can give needles at certain pH at high
PEG400
concentrations.
Next (or at the same time) also seed other protein drops that you
