Exactly, solving the equation is necessary to estimate the
required concentration
Set up the eqn., use large volume (1mL) for soaking solution and
small volume (1microL) for the crystal: therefore C(free) ~ C(total),
simplify, solve for bound ligand in crystal. Thats all.
And I can tell you: st
In those old chemical kinetics courses it was explicitly or
implicitly clear that [I] refers to I(free), not I(total).
The way assays are usually run, [E]<
think about your old chemical kinetics courses.
what counts is concentration and not amount.
M
Dear Marius and others,
here I would like t
think about your old chemical kinetics courses.
what counts is concentration and not amount.
M
> Dear Marius and others,
> here I would like to comment: The problem with soaking is often not
> so much the concentration of the ligand, but the amount of ligand
> needed to fill all binding sites in
