Re: [ccp4bb] A crystallisation screen pH query.

2019-10-06 Thread Bernhard Rupp
works to give an initial idea. LGBR From: CCP4 bulletin board On Behalf Of Jon Cooper Sent: Sunday, October 6, 2019 00:47 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] A crystallisation screen pH query. Many thanks for the excellent replies. I am very happy with the value of 5.1

Re: [ccp4bb] A crystallisation screen pH query.

2019-10-05 Thread Jon Cooper
Many thanks for the excellent replies. I am very happy with the value of 5.1 which two of you kindly sent.Best wishesJon CooperOn 5 Oct 2019 22:51, Bob Cudney wrote: Hello Jon,   That JCSG+ reagent is a copy of Hampton Research PEG/Ion reagent 48.  While there is no pH adjustment made during the

Re: [ccp4bb] A crystallisation screen pH query.

2019-10-05 Thread Peat, Tom (Manufacturing, Parkville)
As was suggested, you can take a small drop and use pH paper to get a rough idea of the pH. The pH will go acidic over time as you have 20% PEG in there. This is an issue with all PEG conditions, so your specific pH will not be known by someone outside your lab as they don't know how old your sc

Re: [ccp4bb] A crystallisation screen pH query.

2019-10-05 Thread Diana Tomchick
Although it sounds as though this condition was not pH adjusted by the company, here are a couple of suggestions that are applicable to ANY commercial screen condition. 1) Try looking at the company's web site for details on how the solution was made, or failing that, try calling the company w