for ground state ATP analogues use AMP-PNP (or AMP-PCP) or ADP in complex
with beryllium fluoride.
In some circumstances ATP-gamma-S will work too. It's less resistant to
hydrolysis, so it's a bad idea for many enzymes, but the resulting active
site geometry can be different depending on whic
Hi Zhiyi,
for ground state ATP analogues use AMP-PNP (or AMP-PCP) or ADP in complex
with beryllium fluoride.
transition state like analogues can be formed with ADP in complex with
aluminium fluoride or magnesium fluoride. ADP - nitrate has also been
used as a transition state analogue, hope this
ent by: CCP4 bulletin board
01/20/2007 12:25 AM Please respond to
CCP4 bulletin board
To
CCP4BB@JISCMAIL.AC.UK cc
Subject
ATP analogues for co-crystallization
Hi everyone,
I want to know which ATP analogues are suitable and popular for
co-crystallization
with enzyme.
Thank you in advance.
>Hey Zhiyi
beta gamma methylene and teta aluminum flourinate derivatives of ATP.
Arti
Hi everyone,
>
> I want to know which ATP analogues are suitable and popular for
> co-crystallization
> with enzyme.
> Thank you in advance.
>
> Best,
> Zhiyi
>
>
>
>
> --
> Zhiyi Wei
Hi, Zhiyi,
Try AMP-PNP, Sigma catalog A2647. Good luck,
Hidong
ZhiYi Wei <[EMAIL PROTECTED]>
Sent by: CCP4 bulletin board
01/20/2007 12:25 AM
Please respond to
CCP4 bulletin board
To
CCP4BB@JISCMAIL.AC.UK
cc
Subject
ATP analogues for co-crystallization
Hi everyo
Hi everyone,
I want to know which ATP analogues are suitable and popular for
co-crystallization
with enzyme.
Thank you in advance.
Best,
Zhiyi
--
Zhiyi Wei
-
Institute of Biophysics, Chinese Academy of Sciences
15 Datu
