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Dear Yu,
I hope you have somebody in your lab to share their expertise with
you, because you will benefit to a much greater extend in learning
data integration from somebody sitting next to you than from the
limited possibilities of online resources.
Dear all,
thank you very much for your kind suggestions.
my crystals are very anisotropic, and usually about 1A between the two
different orientations. here, I post a brief statistic table treated by
HKL2000. In fact, I am mainly working on the crystallization and
characterization of proteins,
I forgot: you should be using FRIEDEL'S_LAW=TRUE
The HKL2000 stats look very "untypical" to me. I wonder what the completeness
is.
For the XDS processing, have you tried the hints given in the Optimization
article in XDSwiki, in particular recycling of GXPARM.XDS and using the
*_E.S.D. parameters as found in INTEGRATE.LP ?
According to your
Dear Yu,
in the CORRECT.LP,
The I/Sig is very low for the resolution higher than 4.17 . If i were
you i probably cut the resolution around 4 Å not 2.8 Å. Because of the
I/sig and CC.
This explain probably the bad electron density. Because, you have not
strong information for the resolution
: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] x-ray diffraction data analysis (XDS)
Dear all,
I recently collected a dataset of a membrane protein. I first processed the
data with HKL2000 to 2.8A ( the diffraction limit) at the beamline, and then
treated the data again with XDS, although I am not
