Re: [ccp4bb] verifying protein crystal content by mass spectrometry or SDS-PAGE

2014-05-02 Thread Bert Van-Den-Berg
, 2014 9:58 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] verifying protein crystal content by mass spectrometry or SDS-PAGE Dear All, Is there any protocol for preparing a sample from (cleaning/dissolving) protein crystals for verifying their mass through mass spectrometry or S

[ccp4bb] verifying protein crystal content by mass spectrometry or SDS-PAGE

2014-05-02 Thread sreetama das
Dear All,    Is there any protocol for preparing a sample from (cleaning/dissolving) protein crystals for verifying their mass through mass spectrometry or SDS-PAGE? How many protein crystals are required? Should they all be from the same well, or can they be from different wells wit