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"LEDU Marie-Helene 16"
Sent by: "CCP4 bulletin board"
18-Nov-2009 03:45
Please respond to "LEDU Marie-Helene 16"
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[ccp4bb] trouble with protein storage
Hello,
Sorry
luck
Thierry
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of LEDU
Marie-Helene 16
Sent: Wednesday, November 18, 2009 03:45 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] trouble with protein storage
Hello,
Sorry for the unrelated questio
You might consider trehalose as a substitute for glycerol.
Cheers,
Roger Rowlett
On 11/18/09, LEDU Marie-Helene 16 wrote:
> Hello,
>
> Sorry for the unrelated question.
> We encounter important storage problem of our favorite protein.
> We can not freeze it without a lost of 50 to 90 %, and
Hmm,
You can try to precipitate it with AmmSO4 and keep it as a suspension at 4C.
Flip
LEDU Marie-Helene 16 wrote:
Hello,
Sorry for the unrelated question.
We encounter important storage problem of our favorite protein.
We can not freeze it without a lost of 50 to 90 %, and can not use g
Hello,
Sorry for the unrelated question.
We encounter important storage problem of our favorite protein.
We can not freeze it without a lost of 50 to 90 %, and can not use glycerol
without precipitation.
Would anybody have suggestions or references that could help us ?
Thanks a lot in advance.
