The paper below can put things in perspective a little bit.
Bernstein and Hol (1997) Acta Crystallogr D Biol Crystallogr. 53(Pt
6):756-64.
Probing the limits of the molecular replacement method: the case of
Trypanosoma brucei phosphoglycerate kinase.
best wishes
Savvas
Sorry - didn't see this - time spent on MR solution of the
Phe-tRNA:EF-Tu:GDPNP structure was about three months (but this was 1994
with slow computers, two trips per year to the synchrotron etc.)
Poul
> To avoid excessive excitement potentially caused by such a list, people
> should also indica
An old example:
The Phe-tRNA:EF-Tu:GDPNP ternary complex - three complexes per asymmetric
unit, C2 space group, degenerate three-fold NCS (three different tRNA
conformations in the trimer). 40 - 2.8 Å resolution native data (about 78%
complete...) used for MR. Straight-forward placement of three EF
To avoid excessive excitement potentially caused by such a list, people
should also indicate the time spent with a model just good enough to
initially justify the eventually futile effort.
Andreas
On 9/21/07, Bryan W. Lepore <[EMAIL PROTECTED]> wrote:
>
> would anyone be willing to share storie
would anyone be willing to share stories of the worst molecular
replacement search probes they used to get the correct solution purely
with MR?
perhaps in terms of %-scattering, RMSD, Z-score, LLG, or other possibly
specific scoring values.
-bryan
