Hi,
Can you explain what you want to do?
Do you just want more of some tRNA corresponding to a codon that is rare in E.
coli you can use the pRare2 plasmid that you can easily isolate from the
Rosetta2 strain (or use Rosetta2 directly if you don't mind).
If you just want some extra tRNA produ
Here's an ignorant question: When people express an exogenous tRNA in E coli
(to overcome rare codon issues, for example, or to supply a cognate tRNA for an
orthogonal synthetase), what sorts of promoters are used?
My (ignorant) guess is that something as potent as the T7 promoter might be a
