Subject: Re: [ccp4bb] spherulites and PEG3350
Something to consider is the quality of the PEG 3350. We have found that
different qualities of PEG 3350 can give different results, depending on the
type and amount of contaminants. What used to be the Fluka PEG 3350 is now the
pharm grade of PEG 3350
her target protein.
Good Luck!
Bryan
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Regina Kettering
Sent: Wednesday, August 24, 2011 2:47 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] spherulites and PEG3350
Something to consider is the quality of the PEG 3350
-
> *From:* Jan van Agthoven
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Sent:* Wednesday, August 24, 2011 2:05 PM
> *Subject:* [ccp4bb] spherulites and PEG3350
>
> Dear all,
>
> I recently obtained some spherulites while trying to crystallize my
> protein. The s
3350 for normal
screening, but switch to the highest quality grade we can get for optimizing.
Regina
From: Jan van Agthoven
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wednesday, August 24, 2011 2:05 PM
Subject: [ccp4bb] spherulites and PEG3350
Dear all,
I recently
You should check these "spherulites" on SDS-PAGE gel to make sure that these
contain your protein. Then you can start thinking about optimization.
On Wed, Aug 24, 2011 at 2:05 PM, Jan van Agthoven wrote:
> Dear all,
>
> I recently obtained some spherulites while trying to crystallize my
> protei
Dear Jan
I would recommend running the following protocol on your spherulites. Just
pretend that they are crystals :)
This was posted some time ago on the ccp4bb.
best regards
Savvas
> On Tue, Nov 2, 2010 at 9:15 AM, Kenneth Verstraete <
> kenneth.verstra...@ugent.be> wrote:
>
> > Hi Ivan,
> >
>
Dear all,
I recently obtained some spherulites while trying to crystallize my protein.
The spherulites are manually reproducible, but changing pH, protein
concentration, and salt concentration does not result in crystal formation.
Microseeding with crushed spherulites isn't a solution either as it
