The best check for what to expect in an asymmetric unit is to run
matthews. It works out the volume of your asymmetric unit, the volume
your molecule should occupy ( based on mol. wt or number of residues)
and indicates whether you could fit one, two and so on molecules into
the cells asymmetr
Your molecule seems to be titled towards at least one of the axis, so there's
no problem with your dimensions in P21.
Jürgen
On Mar 7, 2010, at 7:40 PM, Chunmin L wrote:
> Hi Jürgen,
>
> Many thanks for your message. Somehow Mosflm in CCP4 did not
> recognize the images. Will try XDS. or d*
Hi Jürgen,
Many thanks for your message. Somehow Mosflm in CCP4 did not
recognize the images. Will try XDS. or d*TREK. Still no answers for
that P21 one-molecule solution. It does not collide with itself. The
model is about 60 A wide and 82 A long. Unit cell dimensions of a=50
b=51 c=252. i
On Sunday 07 March 2010, Jürgen Bosch wrote:
>
> On Mar 7, 2010, at 4:35 PM, Chunmin L wrote:
> > I can not reprocess the data to
> > check the systematic absence to determine the space group (I do not
> > have the right software to reprocess the raw image at this time).
>
> What exactly do you
On Mar 7, 2010, at 4:35 PM, Chunmin L wrote:
> I can not reprocess the data to
> check the systematic absence to determine the space group (I do not
> have the right software to reprocess the raw image at this time).
What exactly do you mean by that ? The world does not consist of HKL alone,
t
Hi Dr. Merritt,
Thank you very much for your reply.
>> We are trying to solve a protein structure by molecular replacement.
>> The protein crystal has unit cell dimensions of a=50 b=51 c=252,
>> alpha=90 beta=90.4 gamma=90.
>
>> We processed the data both as P21 (two molecules in A. U.)
>
> OK
>
On Sunday 07 March 2010, Ethan Merritt wrote:
> On Sunday 07 March 2010, Chunmin L wrote:
> > Dear All,
> >
> > We are trying to solve a protein structure by molecular replacement.
> > The protein crystal has unit cell dimensions of a=50 b=51 c=252,
> > alpha=90 beta=90.4 gamma=90.
>
> > We pro
On Sunday 07 March 2010, Chunmin L wrote:
> Dear All,
>
> We are trying to solve a protein structure by molecular replacement.
> The protein crystal has unit cell dimensions of a=50 b=51 c=252,
> alpha=90 beta=90.4 gamma=90.
> We processed the data both as P21 (two molecules in A. U.)
OK
> a
Dear All,
We are trying to solve a protein structure by molecular replacement.
The protein crystal has unit cell dimensions of a=50 b=51 c=252,
alpha=90 beta=90.4 gamma=90. We processed the data both as P21 (two
molecules in A. U.) and P21212 (one molecule in A. U.). We have built
a model based
