[E] richard_robin...@waters.com
-Original Message-
From: CCP4 bulletin board On Behalf Of Markus Heckmann
Sent: 26 April 2018 17:04
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] size exclusion columns
Dear all,
We are looking for a size exclusion chromatography column
(silica-based) for
runtimes of about 10 min per sample with
decent resolution.
Von: CCP4 bulletin board im Auftrag von Artem Evdokimov
Gesendet: Samstag, 28. April 2018 22:16
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] size exclusion columns
Xk10/300 packed with superdex of the
Xk10/300 packed with superdex of the appropriate pore size is by far the
most economical and robust option :) pack your own and save.
Artem
On Thu, Apr 26, 2018, 12:06 Markus Heckmann wrote:
> Dear all,
>
> We are looking for a size exclusion chromatography column
> (silica-based) for protein p
I like the Xbridge columns from Waters, but for SEC-MALS you can also use
Superose 6 Increase (GE), which will take longer to equilibrate and will go
a bit noisier than a silica-based column, but might be advantageous in case
a number of your proteins decide to interact with the silica resin.
Conc
Heckmann
Sent: 26 April 2018 17:04
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] size exclusion columns
Dear all,
We are looking for a size exclusion chromatography column
(silica-based) for protein purification prior to a MALS-detector. We looked for
(www.waters.com BEH-450), Sepax (Unix-C 300) and
Hi Markus,
just be aware that silica-based SEC columns are very sensitive to alkaline pH
conditions, so you should not use them at pH higher that 7.5
We found that to be a limitation and thus chose polymer-based columns.
Hth,
ciao,
Sebastiano
> On 26 Apr 2018, at 18:03, Markus Heckmann wrote:
>
Dear all,
We are looking for a size exclusion chromatography column
(silica-based) for protein purification prior to a MALS-detector. We
looked for (www.waters.com BEH-450), Sepax (Unix-C 300) and Phenomex
(BioZen SEC-3). Any 'column' tips or recommendations when dealing
with large proteins (MDa)
