Re: [ccp4bb] refinement problem of labelled RNA complex

2008-02-18 Thread Manish Chandra Pathak
Hi, It's much easier to begin with the cif file created by Refmac5 itself, but this cif file needs to be checked and corrected manually for the restrains. Certainly, Iodine is not happy with the current restrains. Most of the time, i have found problems beginning with the protonation and th

[ccp4bb] refinement problem of labelled RNA complex

2008-02-18 Thread 侯海峰
Dear all; I am a fresher of Refmac5. And now I'm trying to refine a protein and labelled RNA complex using Refmac. It's a Iodo-U labelled RNA. Unfortunately, these is no appropriate lib file in the current refmac dictionaries. So I use cif file which created by Refmc itself, surprisingly, the b

[ccp4bb] refinement problem of labelled RNA complex

2008-02-18 Thread 侯海峰
Dear all; I am a fresher of Refmac5. And now I'm trying to refine a protein and labelled RNA complex using Refmac. It's a Iodo-U labelled RNA. Unfortunately, these is no appropriate lib file in the current refmac dictionaries. So I use cif file which created by Refmc itself, surprisingly, the b