eGFP, for example, can be fused to the C-
terminal of your target protein. Fluorescent proteins were usually highly
soluble, especially if you select to express in insect cells, which has the
tendency to express proteins in soluble states.
Smith
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Smith Liu
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邮箱：smith_liu...@163.com
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签名
Dear All,Sorry for disturbing you all with my current inquiry. However, I am in
the process of designing and expressing soluble proteins and I thought to ask
for your valuable advice.
1-What would be your first choice (protein-based) fluorophore to link it to the
soluble protein at its C-terminu
