Re: [ccp4bb] recombinant enterokinase

2012-03-15 Thread Wolfgang Skala
Dear Elias, we routinely produce a C-terminally His6-tagged catalytic domain of bovine enterokinase in E. coli. The protease is expressed into inclusion bodies and subsequently folded in vitro. Considering the high activity of our enterokinase construct, one refolding batch allows us to cleave

Re: [ccp4bb] recombinant enterokinase

2012-03-12 Thread Elias Fernandez
Hi All, I misspoke - what I'm looking for is a source for an expression system for recombinant enterokinase. Elias Subject: recombinant enterokinase Dear all, Would anyone know of a source for recombinant enterokinase? Best, Elias

Re: [ccp4bb] recombinant enterokinase

2012-03-12 Thread Sean Seaver
Dear Elias, Here are three sources for recombinant enterokinase: http://www.genscript.com/protein/Z02199-Porcine_Enterokinase_Lyophilized.html http://www.prospecbio.com/Enterokinase/?gclid=CMuXvK6-4a4CFUQUKgodjGwlXQ http://www.neb.com/nebecomm/products/productP8070.asp Hope it helps! Take Care,

Re: [ccp4bb] recombinant enterokinase

2012-03-12 Thread Rajesh kumar
http://www.neb.com/nebecomm/products/productP8070.asp Date: Mon, 12 Mar 2012 09:38:22 -0400 From: elias.fernan...@utk.edu Subject: [ccp4bb] recombinant enterokinase To: CCP4BB@JISCMAIL.AC.UK Dear all,Would anyone know of a source for recombinant enterokinase? Best,Elias

[ccp4bb] recombinant enterokinase

2012-03-12 Thread Elias Fernandez
Dear all, Would anyone know of a source for recombinant enterokinase? Best, Elias