In our protein crystallography course, we succesfully used S-SAD phasing
for proteinase K crystals diffracting to 1.58 Å (which is 'low' for these
crystals) at our home source.
I would highly recommend using proteinase K for heavy atom derivative
demonstration purposes as it seems much easier to
what is it that you want to demonstrate? If it is just for structure
solution, would S-SAD phasing be an option? That way all you need is a
crystal, probably diffracting to better than, say, 2A. And a little bit of
multiplicity.
I assume this is feasible for Lysozyme whereas I don't know the
Hi,
Could anyone give a more or less exact recipee for preparing a
derivate for lysozyme or proteinase K?
like what used, concentration and soak time. we'd need some data sets
for a lab course only thing that
really worked so far was lysozyem KI SIRAS (which however doesnt seem
to be good en
