Re: [ccp4bb] AW: [ccp4bb] question regarding sequence numbering

2017-09-20 Thread John Berrisford
*Von:*CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *Im Auftrag von *Briggs, David C *Gesendet:* Dienstag, 19. September 2017 14:24 *An:* CCP4BB@JISCMAIL.AC.UK *Betreff:* Re: [ccp4bb] question regarding sequence numbering Hi Tony, When I've had similar issues, I've numbered them s

[ccp4bb] AW: [ccp4bb] question regarding sequence numbering

2017-09-19 Thread Herman . Schreuder
. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Briggs, David C Gesendet: Dienstag, 19. September 2017 14:24 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] question regarding sequence numbering Hi Tony, When I've had similar issues, I've num

Re: [ccp4bb] question regarding sequence numbering

2017-09-19 Thread Briggs, David C
quentially, then often graphics and refinement software won't treat them as linked. Dave -- Dr David C Briggs Hohenester Lab Department of Life Sciences Imperial College London UK http://about.me/david_briggs From: Antonio Ariza Sent: Tuesday 19 September, 13:15 Subject: [ccp4bb] question reg

[ccp4bb] question regarding sequence numbering

2017-09-19 Thread Antonio Ariza
Hi all, Here's a problem I haven't come across before. I'm working on a structure whose expression plasmid was designed to remove the first 9 amino acids from the protein of interest and to which an N-terminal tag was added. After cleaving the tag I am left with 3 amino acids (GPM) followed by