Re: [ccp4bb] question about the zinc binding protein

Hi, I'm working with a possible zinc binding protein. Saw your post and was wondering, what is the proper pH range for zinc binding? Thanks, Peter

Re: [ccp4bb] question about the zinc binding protein

I have no idea, it is not my buffer, it is dengzq1987's! 'BTW' means 'by the way', BTW. cheers, charlie >>> Enrico Stura 4/1/2010 11:01 am >>> What is 1mM BTW? I am not familiar with this abbreviation. PBS phoshate buffered saline (Phosphate + NaCl) not suitable for zinc binding proteins

Re: [ccp4bb] question about the zinc binding protein

Behalf Of Enrico Stura Sent: 01 April 2010 11:02 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] question about the zinc binding protein What is 1mM BTW? I am not familiar with this abbreviation. PBS phoshate buffered saline (Phosphate + NaCl) not suitable for zinc binding proteins BBS borat

Re: [ccp4bb] question about the zinc binding protein

What is 1mM BTW? I am not familiar with this abbreviation. PBS phoshate buffered saline (Phosphate + NaCl) not suitable for zinc binding proteins BBS borate buffered saline (Borate + NaCl) wrong pH for zinc binding. BTW ? (? ? ?) No idea what this is. Charles, do you know if it includes HEPE

Re: [ccp4bb] question about the zinc binding protein

Hi, are you sure it is your protein precipitating? You will get a cloudy precipitate appearing in HEPES and Phosphate buffers on addition of ZnCl, without protein. cheers charlie >>> dengzq1987 3/31/2010 5:08 pm >>> hello everyone, recently i purify a protein conteining zinc binding domai

[ccp4bb] question about the zinc binding protein

hello everyone, recently i purify a protein conteining zinc binding domain,and i want to determine its structure.i get the crystal,but poor diffraction.so i try to adding zinc into the protein to optimize the crystal,but the protein precipitate immidiately even the znic is 1 mM.BTW,we use the