What did you see on your ion exchange and gel filtration chromatographs?
ho
Subject: [ccp4bb] question - GFP fusion - cleavage sites
Dear CCP4er's,
Sorry for the non-crystallography related question and was hoping someone on
the bulletin board might have some suggestions to overcome my peculiar protein
purification problem.
I am working on several mem
Hi Celina,
I cannot answer to your question concerning the GFP-related problem.
Fot the thrombin vs TEV protease related question I can tell you that in my
hands thrombin works really very well in most detergents (TEV is somehow
more sensitive). I am working on membrane proteins purified in very
d
Dear CCP4er's,
Sorry for the non-crystallography related question and was hoping someone on
the bulletin board might have some suggestions to overcome my peculiar
protein purification problem.
I am working on several membrane proteins (for crystallization trials) that
have a C-Terminal eGFP fusion
