Re: [ccp4bb] quality homology model

2011-02-08 Thread Robbie Joosten
ss of hydrogen bonds upon folding is quite useful. Just don't expect miracles. Cheers, Robbie Joosten Date: Tue, 8 Feb 2011 10:52:09 -0800 From: eid...@blur.compbio.ucsf.edu Subject: Re: [ccp4bb] quality homology model To: CCP4BB@JISCMAIL.AC.UK Hi Susy, Before

Re: [ccp4bb] quality homology model

2011-02-08 Thread Robbie Joosten
ss of hydrogen bonds upon folding is quite useful. Just don't expect miracles. Cheers, Robbie Joosten Date: Tue, 8 Feb 2011 10:52:09 -0800 From: eid...@blur.compbio.ucsf.edu Subject: Re: [ccp4bb] quality homology model To: CCP4BB@JISCMAIL.AC.UK Hi Susy, Before

Re: [ccp4bb] quality homology model

2011-02-08 Thread Oliv Eidam
Hi Susy, Before going into details: you have to get the alignment right. After that, you may look into packing quality and geometry of your models. Regarding the parameters you are interested in: I talked to a guy from the Sali lab (Modeller) about quality assessment of homology models. He r

[ccp4bb] quality homology model

2011-02-07 Thread Susy Rao
Dear Community, I have a question regarding protein model quality for introduction of point mutations which should increase solibity/stability of my protein of interest. I plan to do homology models so that I can check the most promising amino acids. Which quality/resolution of the model do