Re: [ccp4bb] purification of a strange protein

2010-06-08 Thread Paul Kraft
bject: [ccp4bb] purification of a strange protein To: CCP4BB@JISCMAIL.AC.UK Date: Tuesday, June 8, 2010, 2:13 AM Dear colleagues,     I am now trying to purify a strange protein. The known function of the protein was little, and it was speculated that the protein linked on the surface of cell mem

[ccp4bb] purification of a strange protein

2010-06-07 Thread Oliver Li
Dear colleagues, I am now trying to purify a strange protein. The known function of the protein was little, and it was speculated that the protein linked on the surface of cell membrane. It was expressed with a Trx-tag at very low level of solubility. Furthermore, the protein polymerized