Re: [ccp4bb] protein precipitate in all PEG kit

2012-06-11 Thread Zhijie Li
, one may get better crystallization results. Please read here: http://journals.iucr.org/d/issues/1999/08/00/gr0873/gr0873bdy.html Zhijie From: fengjuanlv12 Sent: Monday, June 11, 2012 3:17 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] protein precipitate in all PEG kit hi all, I got a protein

Re: [ccp4bb] protein precipitate in all PEG kit

2012-06-11 Thread Zhijie Li
: Monday, June 11, 2012 3:17 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] protein precipitate in all PEG kit hi all, I got a protein with good condition without precipitating in the purification process.And the protein is stalbe at 4oC, but when i try crystallization screens, it precipating

[ccp4bb] protein precipitate in all PEG kit

2012-06-11 Thread fengjuanlv12
hi all, I got a protein with good condition without precipitating in the purification process.And the protein is stalbe at 4oC, but when i try crystallization screens, it precipating in all the kit(with PEG), in the kit with high concentration of salt ,the situation was better(but still no crys